What is the assay time for the WIESLAB® Complement System MBL Pathway - RUO?

The estimated assay time is 2h.

What technology and detection system does the WIESLAB® Complement System MBL Pathway - RUO use?

This assay utilizes ELISA technology, with a ALP/pNPP read at 405 nm detection system.

What is the format or size of the WIESLAB® Complement System MBL Pathway - RUO?

This kit is supplied in a 96 wells format.

What is the regulatory status of WIESLAB® Complement System MBL Pathway - RUO?

The regulatory status is Research Use Only.

What is included in the WIESLAB® Complement System MBL Pathway - RUO kit?

The kit includes: One frame with break-apart wells (12x8) ; 35 mL Diluent MP ; 13 mL Conjugate ; 13 mL Substrate solution ; 30 mL Wash solution 30x concentrated ; 0,2 mL Negative control (NC) containing human serum; 0,2 mL Positive control (PC) containing freezed dried human serum; Please note! All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control should be stored at -20° C.

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WIESLAB® Complement System MBL Pathway - RUO

COMPLMP320RUO

RUO ELISA for pathway-specific detection of MBL-mediated complement activation —ideal for preclinical research on innate immunity and infection response.

Reliable, objective, and easy-to-interpret results
Results in just a few hours
Low inter-assay variability and excellent reproducibility
Developed by our in-house experts in collaboration with key opinion leaders
Ready-to-use reagents for a streamlined workflow
Excellent specificity means clear results without false positives

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WIESLAB® Complement System MBL Pathway - RUO

Product Overview

The Wieslab® Complement System MBL pathway kit is an enzyme immunoassay for the qualitative determination of MBL complement pathway and determination of complement deficiencies in human serum. Non-human primate and swine samples can also be assayed with Wieslab Complement System MBL Pathway.

The assay combines the specific activation of each pathway with the use of labeled antibodies specific for C5b-9, a neoepitope of the terminal complement complex produced as a result of complement activation. The amount of C5b-9 generated is proportional to the functional activity of complement pathways.

The Wieslab Complement assays have been published as a useful tool in evaluation of complement therapeutics, in clinic, clinical trials and in drug development.

FOR RESEARCH USE ONLY

This product is also available for in vitro diagnostic use - Product code: COMPL MP320

What's in the box?

One frame with break-apart wells (12x8)
35 mL Diluent MP
13 mL Conjugate
13 mL Substrate solution
30 mL Wash solution 30x concentrated
0,2 mL Negative control (NC) containing human serum
0,2 mL Positive control (PC) containing freezed dried human serum

Please note! All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control should be stored at -20° C.