The CDC (complement-dependent cytotoxicity) mechanism plays a vital role in the immune response by engaging both innate and adaptive immunity. Gaining a deep understanding of CDC is essential for advancing antibody development, especially for engineered antibodies that induce CDC, such as those used in oncology or other therapeutic areas where CDC response is an undesirable side effect.
Additionally, CDC is a critical factor in complement-targeted therapeutic development, as it represents the final step in the classical activation pathway.
It offers a biological significance assay by incorporating Svar LUC, a clever nuclear luciferase construct that remains trapped in the nucleus until cell lysis occurs. This innovative approach enables the detection of even subtle changes in the CDC response.
It presents unparalleled adaptability since it allows for customization by expressing surface antigens of interest to evaluate the cytotoxic effects of a wide variety of antibodies.
It is versatile since it allows you to detect (wanted and unwanted) Ab-mediated cytotoxic effects of therapeutic antibodies with agonistic, antagonistic, or neutralizing functions in functional assays using only one target cell line.
It is well-suited to assess complement targeting therapeutics. By measuring cell lysis induced by complement activation, the assay provides valuable information about the efficacy and potency of complement-targeting therapeutics.
The new CDC bioassay represents a significant leap forward in your therapeutic antibody testing. Its unique reporter gene system offers unparalleled adaptability, simplicity, and sensitivity. With the CDC bioassay, you can confidently measure cell lysis induced by CDC, optimize therapeutic antibody candidates, and drive advancements in your drug development.
Transform your therapeutic development with our Antibody Characterization Assays, including ADCC, ADCP, and the newly introduced CDC, which offer optimal bioassays for assessing the full range of Fc-induced antibody-dependent cytotoxic effects.