iLite® Type I IFN


iLite® Type I IFN Assay Ready Cells are type I IFN responsive reporter gene cells designed for use in assays measuring the functional activity of type I Interferon (IFN) or the presence of neutralizing antibodies (NAbs) against type I IFN.

Due to the functional nature of the assay, both Type I IFN, and any inhibitory agents against Type I IFN or its receptor, can be measured using the same assay.

The iLite Type I IFN cells are human cells (U937) engineered to express Firefly Luciferase under the control of an IFN α/β responsive promoter. When IFNα or IFNβ binds to the IFN α/β receptor on the cell surface, the IFN α/β regulated Firefly Luciferase reporter gene construct will be activated, resulting in a luminescent signal.

The cells can be used for measuring the levels of Type I Interferon (38 samples) or neutralizing antibodies to Type I Interferon (Interferon beta or Interferon alpha) (20 samples).


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iLite® Type I IFN
iLite® Type I IFN

At a glance

Product code BM3049
Assay target Type I IFN
Size 1 vial corresponding to one 96 well plate (1 vial (2.5mL))
Technology Cell-based reporter gene assay
Read-out of results Semi-quantitative,
Assay time 18,5h
Detection system Luminescence
Regulatory status Research Use Only

What's in the box?

2.5 mL of iLite® Type I IFN Assay Ready Cells diluted in RPMI 1640 with 40% heat inactivated fetal bovine serum (FBS), 10% glycerol and 2.5% dimethyl sulfoxide (DMSO).


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