Calprotectin Flexi

Calprotectin is a 36 kilodalton calcium and zinc-binding protein, produced by polymorphonuclear neutrophilic granulocytes, monocytes, and squamous epithelial cells except those in normal skin. The concentration of Calprotectin measured in stools is a non-invasive and objective marker; it can be used to determine disease activity and response to treatment of IBD, and to tell when a true remission has been achieved. Calprotectin can also be analyzed in serum and plasma samples, and high Calprotectin levels have been found in patients with bacterial infections, sepsis, and inflammatory conditions like rheumatoid arthritis (RA) and Systemic Lupus Erythematosus (SLE).

It is recommended to set up Calprotectin Flexi assays as 3-step sandwich assays with the included antibodies in their indicated functions as capture and detector to ensure optimal specificity for detection of native Calprotectin in stool extract or plasma samples. The recommended assay design has also been used in other diagnostic tests developed by Calpro AS (a member of the Svar Life Science group of companies). A recommended Gyrolab^® assay protocol is described below, allowing for automated Calprotectin quantification using Svar Life Science’s proprietary antibodies.

APPLICATIONS for the Calprotectin Flexi Portfolio

This Calprotectin assay and the Calprotectin Flexi reagents can be used to detect Calprotectin in fecal extracts and/or EDTA plasma samples. This application note and the Calprotectin Flexi reagents comprise the essential requirements to independently set up, customize and optimize the Calprotectin assay on the Gyrolab^® automated platform (Gyros Protein Technologies) or another platform of your choice. The Calprotectin Flexi reagents may also be used for other applications of your choice.