What is the assay time for the WIESLAB® Complement System Alternative Pathway - RUO?

The estimated assay time is 2h.

What technology and detection system does the WIESLAB® Complement System Alternative Pathway - RUO use?

This assay utilizes ELISA technology, with a ALP/pNPP read at 405 nm detection system.

What is the format or size of the WIESLAB® Complement System Alternative Pathway - RUO?

This kit is supplied in a 96 wells format.

What is the regulatory status of WIESLAB® Complement System Alternative Pathway - RUO?

The regulatory status is Research Use Only.

What is included in the WIESLAB® Complement System Alternative Pathway - RUO kit?

The kit includes: One frame with break-apart wells (12x8) ; 35 mL Diluent AP; 13 mL Conjugate ; 13 mL Substrate solution ; 30 mL Wash solution 30x concentrated ; 0,2 mL Negative control (NC) containing human serum; 0,2 mL Positive control (PC) containing freezed dried human serum; Please note: All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control must be stored at -20°C.

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WIESLAB® Complement System Alternative Pathway - RUO

COMPLAP330RUO

Research-use-only ELISA for profiling alternative pathway activation—designed for in-depth studies of complement-mediated mechanisms and screening of investigational compounds.

Reliable, objective, and easy-to-interpret results
Results in just a few hours
Low inter-assay variability and excellent reproducibility
Developed by our in-house experts in collaboration with key opinion leaders
Ready-to-use reagents for a streamlined workflow
Excellent specificity means clear results without false positives

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WIESLAB® Complement System Alternative Pathway - RUO

Product Overview

The Wieslab® Complement System Alternative Pathway kit is an enzyme immunoassay for the qualitative determination of functional alternative complement pathway and for the determination of complement deficiencies in human serum. Non-human primate and swine samples can also be assayed with Wieslab® Complement System Alternative pathway.

The Wieslab Complement assay combines the specific activation of the pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. The amount of C5b-9 generated is proportional to the functional activity of the complement system. The convenient ELISA technology has several advantages compared to the traditional hemolytic assays.

The Wieslab  Complement assays have been reported in literature as useful tools in the evaluation of complement therapeutics, in clinic, clinical trials and in drug development.

FOR RESEARCH USE ONLY

This product is also available for in vitro diagnostic use - Product code: COMPL AP330.

What's in the box?

One frame with break-apart wells (12x8)
35 mL Diluent AP
13 mL Conjugate
13 mL Substrate solution
30 mL Wash solution 30x concentrated
0,2 mL Negative control (NC) containing human serum
0,2 mL Positive control (PC) containing freezed dried human serum

Please note: All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control must be stored at -20°C.